Ethanolic Extract of Sapindus Mukorrossi as Final Endodontic Irrigant.

Summary

The chief aim of root canal treatment is to reduce the microbial content and prevent further recontamination in the canal. Properly cleaned, shaped and obturated teeth will be having fewer chances of treatment failure. Every tooth has variable, complex canal anatomy consisting of ramifications and lateral canal. The instrumentation alone cannot reach all the areas for cleaning. For proper cleaning and removal of all microorganisms, the canal should be irrigated with any biocompatible, lubricant and antimicrobial agent. An irrigant should have a high compatibility factor, with canals, with obturating material and sealer. It should remove all the superficial barriers from canal tubules and allow sealer penetration, which aids in better adhesion and decreases the chances for recontamination. Objectives: This study aims to compare the effect of experimental endodontic irrigant Sapindus mukorossi with 17% EDTA, on the microleakage, sealer penetration and interaction with sodium hypochlorite. Ninety single-rooted teeth will be allocated into 2 experimental groups and a control group of 30 teeth each. The endodontic procedure will be done with protaper files. The sample in group 1 will be irrigated with 3.0% NaOCl after that by 17% EDTA and the sample in group 2 final irrigation will be given with Ethanolic extract of Sapindus mukorossi. After the preparation and final irrigation of all teeth, sterile saline will be used to flush all canals and dried using paper points. Obturation will be done with lateral condensation by gutta-percha. Teeth will be incubated at 37ºc and 100% humidity for 7 days. Varnish will be applied on the root surface except for apical 3mm. The teeth will be sectioned longitudinally and examined at 2 and 5mm from the root apex. The maximum depth of sealer penetration will be measured using a scanning electron microscope and microleakage will be tested through a dye penetration method under a stereomicroscope. Testing of interaction between irrigant and hypochlorite will be done by placing 1ml irrigant with 1 ml sodium hypochlorite in a round bottom test tube and left for 1 week at 37ºc. An independent observer will look for precipitation formation or color changes

Timeline

Start

2020-04-02

Primary completion

2021-06-03

Completion

2021-09-01

Publications

• Background Thaha KA, Varma RL, Nair MG, Sam Joseph VG, Krishnan U. Interaction between Octenidine-based Solution and Sodium Hypochlorite: A Mass Spectroscopy, Proton Nuclear Magnetic Resonance, and Scanning Electron Microscopy-based Observational Study. J Endod. 2017 Jan;43(1):135-140. doi: 10.1016/j.joen.2016.09.015. Epub 2016 Dec 6.
• Background Pommel L, About I, Pashley D, Camps J. Apical leakage of four endodontic sealers. J Endod. 2003 Mar;29(3):208-10. doi: 10.1097/00004770-200303000-00011.
• Background Farhad AR, Barekatain B, Koushki AR. The Effect of Three Different Root Canal Irrigant Protocols for Removing Smear Layer on the Apical Microleakage of AH26 Sealer. Iran Endod J. 2008 Summer;3(3):62-7. Epub 2008 Jul 10.
• Background Violich DR, Chandler NP. The smear layer in endodontics - a review. Int Endod J. 2010 Jan;43(1):2-15. doi: 10.1111/j.1365-2591.2009.01627.x.
• Background Bloomfield SF, Miles GA. The antibacterial properties of sodium dichloroisocyanurate and sodium hypochlorite formulations. J Appl Bacteriol. 1979 Feb;46(1):65-73. doi: 10.1111/j.1365-2672.1979.tb02582.x. No abstract available.
• Background Kuci A, Alacam T, Yavas O, Ergul-Ulger Z, Kayaoglu G. Sealer penetration into dentinal tubules in the presence or absence of smear layer: a confocal laser scanning microscopic study. J Endod. 2014 Oct;40(10):1627-31. doi: 10.1016/j.joen.2014.03.019. Epub 2014 May 10.

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