The Role of the Gut Microbiota in the Systemic Immune Response During Human Endotoxemia

Summary

The purpose of this study is to determine whether treatment with antibiotics, which harm the gut flora, causes the immune system to be less effective.

Timeline

Start

2014-06

Primary completion

2015-12

Completion

2015-12

Outcome

Missed primary endpoint

paper Clinical parameters, neutrophil influx, cytokine production, coagulation activation and endothelial activation during endotoxemia were not different between antibiotic-pretreated and control individuals. PMID 27307305 ↗

paper These findings suggest that gut microbiota disruption by broad-spectrum antibiotics does not affect systemic innate immune responses in healthy subjects during endotoxemia in humans PMID 27307305 ↗

Publications

• Haak BW, Lankelma JM, Hugenholtz F, Belzer C, de Vos WM, Wiersinga WJ. Long-term impact of oral vancomycin, ciprofloxacin and metronidazole on the gut microbiota in healthy humans. J Antimicrob Chemother. 2019 Mar 1;74(3):782-786. doi: 10.1093/jac/dky471.
• Lankelma JM, Cranendonk DR, Belzer C, de Vos AF, de Vos WM, van der Poll T, Wiersinga WJ. Antibiotic-induced gut microbiota disruption during human endotoxemia: a randomised controlled study. Gut. 2017 Sep;66(9):1623-1630. doi: 10.1136/gutjnl-2016-312132. Epub 2016 Jun 15.

Drugs

Evaluation	Drug	Modality	Dose	Route
Subject	Ciprofloxacin	Small molecule	500 mg	Oral
Subject	Metronidazole	Small molecule	500 mg	Oral
Subject	Vancomycin	Other / unclassified	500 mg	Oral

Indications

No indication recorded.