Molecular Analysis of Intracanal Microbes After Chemomechanical Procedure and Root Canal Medication: A Randomized Controlled Trial

Phase 1 Completed 20 enrolled Mansoura University

RandomizedParallel-groupSingle-blindTreatment

Summary

This study aimed to evaluate clinically the intracanal microbes at molecular level after using established root canal medicament Calcium hydroxide and potential root canal medicament Glycyrrhizin.and Decontamination Analysis of Controls samples.

Timeline

Start: 2022-05-03
Primary completion: 2023-12-20
Completion: 2024-01-15

Outcome

Met primary endpoint

paper The reduction in log‑transformed RNA (primary end-point) and DNA from S2 to S3 (Δlog S3–S2) was significantly greater in Group 2 than in Group 1 PMID 41814304 ↗

paper RNA: p < 0.0001 PMID 41814304 ↗

Publications

Khaleefah OT, El-Baz AM, Gomaa MA, Badr AE. Molecular analysis of intracanal microbial reduction to compare the effectiveness of glycyrrhizin and calcium hydroxide as intracanal medications: a randomised controlled trial. BMC Oral Health. 2026 Mar 11;26(1):731. doi: 10.1186/s12903-026-07906-6.

Drugs

Evaluation	Drug	Modality	Dose	Route
Subject	Glycyrrhizin	Unknown	—	—