Drugs / dornase alfa
last change May 2026 re-read 2 minutes ago

dornase alfa

Protein / enzyme biologic

Developed for
COVID-19 · cystic fibrosis · pleural empyema · acute respiratory distress syndrome · ischemic stroke · sinusitis · adult acute respiratory distress syndrome · brain ischemia
+17 more · cancer · chronic rhinosinusitis · disease related to solid organ transplantation · empyema · germ cell tumor · head and neck cancer · large B-cell lymphoma · pneumonia · respiratory tract infectious disorder · dry eye syndrome · graft versus host disease · inflammatory disease · lung disorder · otitis media · pleural disorder · respiratory failure · Sjogren syndrome
Investigated by
Genentech, Inc. · Fondation Ophtalmologique Adolphe de Rothschild · Hoffmann-La Roche · University Hospital, Strasbourg, France · AO GENERIUM · Acibadem University
+59 more · Azienda Ospedaliera di Padova · Boston Children's Hospital · Brigham and Women's Hospital · British Columbia Children's Hospital · Canadian Institutes of Health Research (CIHR) · Centre Hospitalier Régional Metz-Thionville · Children's Hospital of Eastern Ontario · DHR Health Institute for Research and Development · Dartmouth-Hitchcock Medical Center · Dr Carey Denholm and Laura Denholm · Eastern Regional Medical Center · Emory University · Exactis Innovation · Georgetown University · Hamilton Health Sciences Corporation · Joachim Riethmueller, Dr. med. · Jon Simmons · Long Beach Memorial Medical Center · M.D. Anderson Cancer Center · Main Line Health · McGill University Health Centre/Research Institute of the McGill University Health Centre · McMaster Children's Hospital · Milton S. Hershey Medical Center · National Cancer Institute, Slovakia · National University of Malaysia · Northwestern University · Prisma Health-Midlands · QuantumLeap Healthcare Collaborative · South Shore Hospital · St. Justine's Hospital · Swedish Medical Center · Tel-Aviv Sourasky Medical Center · The Alfred · The Alfred Research Trusts Small Project Grant. · The Hospital for Sick Children · The Physicians' Services Incorporated Foundation · The Scientific and Technological Research Council of Turkey · Unity Health Toronto · University College, London · University Hospital Tuebingen · University Hospital, Tours · University Hospitals Cleveland Medical Center · University Medical Centre Ljubljana · University of Alabama at Birmingham · University of California, Davis · University of California, Irvine · University of California, San Francisco · University of Colorado, Denver · University of Jena · University of Melbourne · University of Miami · University of Michigan · University of Missouri-Columbia · University of South Alabama · University of Southern California · University of Vermont · Wake Forest University Health Sciences · West Virginia University · Yale University

Regulatory milestones approvals, filings & regulatory actions · 4 recorded

MilestoneJurisdictionBrandIndicationDateSentence it was read from
Label expansions (3) US (FDA) PULMOZYME 1996-11-27 – 2014-12-12 fda.gov
Approved US (FDA) PULMOZYME 1993-12-30 fda.gov

Trials 32 · a red edge is where a trial was stopped

PhaseRegistry idDatesIndicationSponsorStatusOutcome
Phase 01 trial
Phase 0 NCT00536952 Feb 2008 → Oct 2014 head and neck cancer Northwestern University Unknown No outcome recorded
Phase 13 trials
Phase 1 NCT07361224 May 2026 → Feb 2027 expected large B-cell lymphoma Tel-Aviv Sourasky Medical Center Recruiting No outcome recorded
Phase 1 NCT04409925 Dec 2020 → Apr 2021 COVID-19 McGill University Health Centre/Research Institute of the McGill University Health Centre Completed No outcome recorded
Phase 1 NCT02135588 Oct 2013 → Dec 2021 cancer Eastern Regional Medical Center Unknown No outcome recorded
Phase 211 trials
Phase 2 NCT06723717 Sep 2025 → Mar 2028 expected acquired aneurysmal subarachnoid hemorrhage, brain ischemia Fondation Ophtalmologique Adolphe de Rothschild Recruiting No outcome recorded
Phase 2 NCT07121322 Jul 2025 → Jul 2027 expected germ cell tumor National Cancer Institute, Slovakia Recruiting No outcome recorded
Phase 2 NCT04785066 Aug 2022 → Aug 2025 ischemic stroke Fondation Ophtalmologique Adolphe de Rothschild Completed No outcome recorded
Phase 2 NCT05203224 Feb 2022 → Nov 2025 ischemic stroke University of Melbourne Completed No outcome recorded
Phase 2 NCT04488081 Jul 2020 → Jul 2028 expected COVID-19 QuantumLeap Healthcare Collaborative Active not recruiting No outcome recorded
Phase 2 NCT04402944 Jul 2020 → Dec 2021 COVID-19 Boston Children's Hospital Unknown No outcome recorded
Phase 2 NCT04359654 Jun 2020 → Aug 2021 COVID-19 University College, London Completed No outcome recorded
Phase 2 NCT04432987 May → Aug 2020 COVID-19 Acibadem University Unknown No outcome recorded
Phase 2 NCT04445285 Apr 2020 → Nov 2021 overdue COVID-19 Jon Simmons Recruiting No outcome recorded
Phase 2 NCT01952470 Oct 2013 → Aug 2017 disease related to solid organ transplantation, respiratory tract infectious disorder The Alfred Completed No outcome recorded
Phase 2/32 trials
Phase 2/3 NCT00502632 Oct 2007 → Oct 2009 pleural empyema Azienda Ospedaliera di Padova Unknown No outcome recorded
Phase 2/3 NCT00204685 Aug 2003 → Jul 2006 University Hospital Tuebingen Unknown No outcome recorded
Phase 37 trials
Phase 3 NCT04402970 Jun → Dec 2020 COVID-19, acute respiratory distress syndrome University of Missouri-Columbia Completed No outcome recorded
Phase 3 NCT04459325 Jun → Jul 2020 COVID-19 AO GENERIUM Completed No outcome recorded
Phase 3 NCT04355364 Apr 2020 → Dec 2021 COVID-19, acute respiratory distress syndrome Fondation Ophtalmologique Adolphe de Rothschild Terminated No outcome recorded
Phase 3 NCT03368092 Mar 2019 → Sep 2027 expected adult acute respiratory distress syndrome University Hospital, Strasbourg, France Recruiting No outcome recorded
Phase 3 NCT01155752 Jul 2013 → Jun 2014 cystic fibrosis, sinusitis Milton S. Hershey Medical Center Withdrawn No outcome recorded
Phase 3 NCT01717742 Dec 2012 → Dec 2017 pleural empyema The Hospital for Sick Children Completed No outcome recorded
Phase 3 NCT00534079 Sep 2007 → Feb 2009 chronic rhinosinusitis, cystic fibrosis University of Jena Completed No outcome recorded
Phase 47 trials
Phase 4 NCT07592520 Apr 2026 → Apr 2027 expected pleural empyema Dartmouth-Hitchcock Medical Center Not yet recruiting No outcome recorded
Phase 4 NCT05139901 Dec 2021 → Feb 2023 pneumonia University Medical Centre Ljubljana Terminated No outcome recorded
Phase 4 NCT03873766 Apr 2019 → Mar 2022 empyema, pleural empyema Swedish Medical Center Completed No outcome recorded
Phase 4 NCT03167281 Feb 2017 → Mar 2021 Prisma Health-Midlands Withdrawn No outcome recorded
Phase 4 NCT00843817 Apr 2009 → Apr 2013 cystic fibrosis University Hospital, Tours Completed No outcome recorded
Phase 4 NCT00680316 Jun 2008 → May 2009 cystic fibrosis Genentech, Inc. Terminated No outcome recorded
Phase 4 NCT00434278 Mar 2007 → Nov 2008 cystic fibrosis Genentech, Inc. Terminated No outcome recorded
Phase not applicable1 trial
NCT04915586 Dec 2019 → Dec 2020 pleural empyema National University of Malaysia Completed No outcome recorded
Also used as a comparator or background therapy in 17 trials
PhaseRegistry idDatesIndicationSponsorStatusOutcome
Phase 12 trials
Phase 1 NCT03586076 Comparator Jan → Jun 2018 JHL Biotech, Inc. Completed No outcome recorded
Phase 1 NCT01357447 Comparator May 2011 → Mar 2012 Sjogren syndrome UConn Health Withdrawn No outcome recorded
Phase 1/22 trials
Phase 1/2 NCT02702518 Comparator Apr 2016 → Apr 2020 dry eye syndrome, graft versus host disease University of Illinois at Chicago Terminated No outcome recorded
Phase 1/2 NCT02193490 Comparator Jul 2014 → Oct 2017 dry eye syndrome University of Illinois at Chicago Terminated No outcome recorded
Phase 22 trials
Phase 2 NCT05880524 Comparator Dec 2024 → Dec 2025 inflammatory disease, ischemic stroke Ludwig-Maximilians - University of Munich Unknown No outcome recorded
Phase 2 NCT00117208 Comparator Nov 2005 → Feb 2008 cystic fibrosis Syntara Completed No outcome recorded
Phase 32 trials
Phase 3 NCT04468100 Comparator Aug 2017 → May 2018 cystic fibrosis AO GENERIUM Completed No outcome recorded
Phase 3 NCT00265434 Comparator Dec 2005 → May 2006 chronic rhinosinusitis, cystic fibrosis University of Jena Completed No outcome recorded
Phase 45 trials
Phase 4 NCT03213834 Comparator Sep 2017 → Feb 2020 pleural disorder University of Florida Completed No outcome recorded
Phase 4 NCT01712334 Comparator Dec 2012 → Jun 2013 cystic fibrosis Genentech, Inc. Completed No outcome recorded
Phase 4 NCT01356147 Comparator May 2011 → Jan 2015 respiratory tract infectious disorder Georgetown University Completed No outcome recorded
Phase 4 NCT02168387 Comparator Dec 2010 → May 2012 lung disorder Duke University Completed No outcome recorded
Phase 4 NCT00419380 Comparator Jan 2007 → May 2011 otitis media University of Colorado, Denver Completed No outcome recorded
Phase not applicable4 trials
NCT05903417 Comparator Jul 2023 → Oct 2025 respiratory tract infectious disorder National University of Malaysia Completed No outcome recorded
NCT05279391 Comparator Oct 2020 → Nov 2021 respiratory failure Democritus University of Thrace Unknown No outcome recorded
NCT00671723 Comparator Oct 2006 → Jul 2008 lung disorder University of Oklahoma Completed No outcome recorded
NCT01095276 Comparator Nov 2005 → Jan 2006 lung disorder University of Medicine and Dentistry of New Jersey Completed No outcome recorded

Evidence & citations 17 cited values

Every value below carries the sentence it was read from. 54 sources stand behind the page.

FieldValueCited text
Known as dornase alfa “Though intraplerual alteplase/ dornase alfa and normal saline have been established method to reduce complicated pleural infection sizes and rate of surgical interventions,...” NCT07592520
30

Dornase alfa, a recombinant DNAse 1, has been proposed to attenuate these effects by degrading extracellular DNA and enhancing alveolar clearance of NETs.” PMID 41125713 Oct 2025

“The aqueous solution contains 1.0 mg/mL dornase alfa, 0.15 mg/mL calcium chloride dihydrate, and 8.77 mg/mL sodium chloride.” NCT01155752

“Nasal Inhalation of Dornase Alfa (Pulmozyme) in Patients With Cystic Fibrosis and Chronic Rhinosinusitis” NCT00265434

“Nebulised dornase alfa \[2.5 mg BID\] for 7 days” NCT04459325

“intrapleural dornase alfa and tPA administered” NCT02135588

NCT03586076

NCT04355364

NCT05880524

NCT05139901

NCT00204685

NCT00680316

NCT01712334

NCT05203224

NCT04359654

NCT00434278

NCT00502632

NCT00534079

NCT04785066

NCT03368092

NCT01356147

NCT01952470

NCT01357447

NCT07121322

NCT02135588

NCT05279391

NCT00419380

NCT06723717

NCT04488081

NCT02168387

NCT04402970

Known as deoxyribonuclease I “Dornase alfa is a recombinant human deoxyribonuclease I (DNase I) approved for use in cystic fibrosis, where it enzymatically degrades extracellular DNA and reduces mucus viscosity.” NCT07121322
1

“Recombinant Deoxyribonuclease I Eye Drops for Ocular Graft Versus Host Disease: Results of a Randomized Clinical Trial.” PMID 38407974 Feb 2024

Known as DNAase “clinical trial using deoxyribonuclease I (DNAase) eye drops to test the hypothesis that reducing the abundance of NETs from the ocular surface will reduce signs and symptoms of oGVHD.” PMID 38407974 Feb 2024
Known as DNase “Patients randomized to the IPFT study arm will receive a total of 5-6 doses of alteplase 10mg and DNase 5 mg BID x 3 days delivered through a chest tube or small bore catheter...” NCT03873766
7

“intrapleural 10 mg alteplase (t-PA) with 5 mg of pulmozyme (DNase) twice daily for 72 h were used.” PMID 35581627 May 2022

“Combination TISSUE PLASMINOGEN ACTIVATOR (TPA) AND DORNASE ALFA (DNASE)” NCT02135588

“t-PA (Alteplase) 5mg and DNase (Pulmozyme)5mg” NCT05903417

NCT03167281

NCT03213834

NCT01717742

NCT02193490

Known as DNAse alfa DNAse alfa (Pulmozyme®, Roche) is a recombinant human enzyme” NCT05139901
Known as DNase I “Dornase alfa is a recombinant human deoxyribonuclease I (DNase I) approved for use in cystic fibrosis, where it enzymatically degrades extracellular DNA and reduces mucus viscosity.” NCT07121322
Known as dornase “The purpose of this study is to evaluate Pulmozyme® (dornase) as compared to a standard ear drop Floxin® (ofloxicin) to dissolve clogged tubes.” NCT00419380
Known as Dornase alfa recombinant human deoxyribonuclease I (rhDNase) Dornase alfa recombinant human deoxyribonuclease I (rhDNase)NCT01155752
Known as dornase alpha “Recombinant human deoxyribonuclease I (rhDNase, dornase alpha), administered by inhalation, is currently used as a mucolytic agent in the treatment of cystic fibrosis.” NCT00204685
4

“PULMOZYME contains an active substance called dornase alpha as an active substance.” NCT04432987

NCT00671723

NCT01095276

NCT00117208

Known as PULMOZYME “Dornase alfa (Pulmozyme®, Roche, Switzerland) will be administered by aerosol, at a dose of 2500 IU twice daily, 12 hours apart, for 7 consecutive days, using a vibrating mesh...” PMID 32560746 Jun 2020
20

“Evaluation of the Efficacy of Dornase Alfa (Pulmozyme®) on Arterial Recanalization in Post-thrombectomy Angiography in Patients With Ischemic Stroke by Thrombolysis and...” NCT04785066

“2.5 mg of DNase (Dornase alpha, PULMOZYME® , Genentech, South San Francisco, CA), nebulized twice daily, after a 15 min premedication with nebulized albuterol (2.5 mg diluted...” NCT00671723

“Dornase alfa (Pulmozyme®, Roche 2500U, 2,5mL) given by aerosol in the respiratory circuit (Aerogen solo®) within 6h at day 1 and 24 hours after on day 2.” NCT03368092

“The purpose of this study is to evaluate Pulmozyme® (dornase) as compared to a standard ear drop Floxin® (ofloxicin) to dissolve clogged tubes.” NCT00419380

“In humans, recombinant DNAse (dornase alfa, Pulmozyme®) has marketing authorization for inhaled administration in cystic fibrosis.” NCT06723717

“DNAse alfa (Pulmozyme®, Roche) is a recombinant human enzyme” NCT05139901

“t-PA (Alteplase) 5mg and DNase (Pulmozyme)5mg” NCT05903417

“Dornase Alfa Inhalation Solution [Pulmozyme]” NCT04359654

“dornase alfa [Pulmozyme®]” NCT01712334

“Dornase alfa (Pulmozyme)” NCT00534079

PulmozymeNCT04402970

NCT04915586

NCT04468100

NCT00843817

NCT00265434

NCT04445285

NCT04432987

NCT00536952

NCT04402944

NCT00416182

Known as Recombinant human deoxyribonuclease (rh-DNase) “Patient will receive 2.5mg Pulmozyme/ Recombinant human deoxyribonuclease (rh-DNase) aerosolized treatment once every 24 hours for five (5) consecutive days; a total of five (5) doses.” NCT04445285
Known as Recombinant human deoxyribonuclease I Recombinant human deoxyribonuclease I (rhDNase, dornase alpha), administered by inhalation, is currently used as a mucolytic agent in the treatment of cystic fibrosis.” NCT00204685
Known as rhDNase “A Phase I/II Randomized Placebo-Controlled, Double-Blind, Single-Center, Tolerability and Preliminary Efficacy Clinical Trial of Recombinant Human Deoxyribonuclease (rhDNase)...” NCT02702518
2

“Patient will receive 2.5mg Pulmozyme/ Recombinant human deoxyribonuclease (rh-DNase) aerosolized treatment once every 24 hours for five (5) consecutive days; a total of five (5) doses.” NCT04445285

“Recombinant human deoxyribonuclease I (rhDNase, dornase alpha), administered by inhalation, is currently used as a mucolytic agent in the treatment of cystic fibrosis.” NCT00204685

Known as rhDNase I ClinicalTrials.gov intervention name — accepted as the source's own label NCT07361224
2

NCT04409925

NCT02702518

Known as rhDNase, pulmozyme rhDNase, pulmozymeNCT00117208
Modality Protein / enzyme biologic “recombinant human deoxyribonuclease (rhDNase), the current best established mucolytic treatment” PMID 19996349 Dec 2009
Route Inhaled “nebulised rhDNase alone” PMID 19996349 Dec 2009